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SEMINAR 1: "Cryo-transmission X-ray microscopy" by Liz Duke, Diamond, UK
Latest News
: SEMINAR 1: "Cryo-transmission X-ray microscopy" by Liz Duke, Diamond, UK
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SEMINAR 1: "Cryo-transmission X-ray microscopy" by Liz Duke, Diamond, UK
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Thr, February 25, 2010
at 3pm
GAC
1130
ConfRoom
4DB Physics Oncology Seminar Series
SEMINAR 1:
Liz Duke, Diamond, UK
Cryo-transmission X-ray microscopy: Is it worth it?
For many years the tool of choice for the study of cellular structure at the supramolecular level has been electron microscopy with recent advances allowing 3D electron tomograms to be created. However, despite achieving some spectacular results, this technique is not without its drawbacks and challenges.
In contrast, some 50+ years ago X-ray microscopy was heralded as being THE imaging technique as all its inherent difficulties had been solved. If this were the case, why isn’t a full field microscope found in every home laboratory and each synchrotron equipped with half a dozen X-ray microscopy beamlines – following the trend well established in macromolecular crystallography?
More recently a few small changes in the landscape of biological full field X-ray microscopy have been seen with increasing amounts of biological work being carried out on the BESSY beamline and a new beamline dedicated to biology coming on line at the ALS. Combine this with a new cryo-TXM facility which will start commissioning this year at the new Spanish synchrotron, ALBA and a completely new beamline at the design stage at the UK synchrotron, Diamond and possibly we are looking at a whole new ball game? But why this change in attitude to the technique?
In this talk I will consider the pros and cons of the various 3D imaging methods and present the case for cryo-transmission X-ray microscopy in order to put forward a considered response to the question posed in the title: “Cryo-transmission X-ray microscopy: Is it worth it?”
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2/25/2010
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Created at 2/11/2010 1:58 PM by Katya Kadyshevskaya
Last modified at 2/19/2010 1:30 PM by Katya Kadyshevskaya
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