Latest News - SEMINAR 2:"Immuno-magnetic enrichment and...

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SEMINAR 2:"Immuno-magnetic enrichment and flow cytometric isolation of circulating and disseminated tumor cells for genomic characterization" by Mark Magbanua, UCSF

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Thursday, February 25, 2010

at 4:15pm

GAC1130 ConfRoom

4DB Physics Oncology Seminar Series

Seminar 2:

Mark Magbanua, UCSF
Immunomagnetic enrichment and flow cytometric isolation of circulating and disseminated tumor cells for genomic characterization

ABSTRACT

Molecular characterization of circulating tumor cells (CTCs) from blood and disseminated tumor cells (DTCs) from bone marrow is technically challenging because cells are rare and difficult to isolate. We developed a novel procedure to isolate tumor cells with high purity from blood or bone marrow via immunomagnetic enrichment followed by fluorescence activated cell sorting (IE/FACS). DNA from small pools of isolated tumor cells was subjected to copy number analysis via array comparative genomic hybridization following whole genome amplification. Proof of principle experiments performed on isolated BT474 cells spiked into normal blood correctly identified the known genomic alteration. In clinical studies, array CGH analyses of CTCs and DTCs revealed significant genomic aberrations. Common copy number alterations included loss of 8p and gains in 8q. The magnitude of measured copy number changes indicated essentially no hematopoietic cell contamination. Comparison of primary tumor and CTCs confirmed shared genomic aberrations. We also demonstrate the feasibility and reproducibility of serial genomic analyses of CTCs.

4DB Center: Physics of Metastasis over Time and Space

Latest News: SEMINAR 2:"Immuno-magnetic enrichment and flow cytometric isolation of circulating and disseminated tumor cells for genomic characterization" by Mark Magbanua, UCSF

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